Phytochemical Screening and Acute Exposure of Methanolic Bark Extract of Pterocarpus Tinctorius in Wistar Rats
Keywords:
Pterocarpus tinctorius, toxicity, phytochemical screening, Wistar rats.
Abstract
The bark extract of Pterocarpus tinctorius (Fabaceae) is traditionally used to treat diseases such as diabetes, gonorrhoea, hypertension, stomachache and bacterial gastroenteritis. However, little is known about toxicity and phytochemicals found in the bark of Pterocarpus tinctorius. Therefore, this study aimed at phytochemical screening and investigating acute oral toxicity of the stem bark of Pterocarpus tinctorius in Wistar rats. Wistar rats (N=3 per group) were randomly assigned to four groups: negative control, 50, 300, and 2000 mg/ kg methanolic sub-extract. A single dose was administered and female Wistar rats were observed for 14 days according to the Organisation for Economic Co- operation and Development guidelines for acute toxicity testing in rats. After dosing, rats were individually observed for their physical and behavioural changes throughout the study. The body weight of Wistar rats were monitored weekly and gross pathological changes of rat organs were observed. In addition, the biochemical markers and organ- body weight ratios of the kidneys and the liver were measured. Qualitative phytochemical tests were carried out to determine the types of phytochemicals present in the bark of Pterocarpus tinctorius. There were no signs of toxicity observed in all treatment groups and no abnormalities were observed on organs of rats. There was no significant difference in body weight, organ- body weight and biochemical markers with p>0.05, for the Wistar rats in all treatment groups compared to the negative control. Phytochemical tests showed the presence of alkaloids, phenolics, flavonoids, tannins, steroids, terpenoids, anthocyanins and saponins in the stem bark of Pterocarpus tinctorius as compounds that could be responsible to treat diseases in traditional medicine. This study demonstrated that Pterocarpus tinctorius methanolic bark sub-extract is not acutely toxic to the liver and kidneys up to the dose of 2000 mg/kg body weight. It is highly recommended that toxicity studies on other organs of rats such as the heart, brain, pancreas and intestines are carried out.References
1. Dickson A M, Fred O N and Eleojo O: Phytochemical, antibacterial and toxicity studies of the aqueous extract of Eucalyptus camaldulensis Dehnh. Asian Journal of Plant Science and Research 2011; 1 (3), 1-10.
2. Nasri H and Shirzad H: Toxicity and safety of medicinal plants. Journal of HerbMed Pharmacology 2013; 2(2): 21-22.
3. Botha CJ and Penrith ML: Poisonous plants of veterinary and human importance in Southern Africa, Journal of Ethnopharmacology 2008; 119, 549-58.
4. Kalaiselvi P, Uma M, Suresh M, Thulasiraman K and Lakshmidevi E: Chronic toxicity studies of aqueous leaf extract of Indian traditional medicinal plant Ocimum tenuiflorum (Linn.) in rats European. Journal of Experimental Biology 2013; (5): 240-247.
5. Fred-Jaiyesimi A A, Ajibesin K K: Ethnobotanical survey of toxic plants and plant parts in Ogun State, Nigeria. International Journal of Green Pharmacy 2012; 6: 174-9.
6. Atchou K, Lawson-Evi P and Eklu- Gadegbeku K: Safety assessment of the dried hydroethanolic extracts of Pterocarpus erinaceus Poir. Stem bark, Phytomedicine Plus 2021; 1(4).
7. Salawu O A, Aliyu M and Tijani AY: Hematological studies on the ethanolic stem bark extract of Pterocarpus erinaceous Poir (Fabaceae). African Journal of Biotechnology 2008; 7(9).
8. Chanda J, Parekh J, Vaghasiya Y, Dave R, Baravalia Y and Nair R: Medicinal plants- from-from Traditional use to toxicity assessment: A Review- International Journal of Pharmaceutical Sciences and Research 2015; 6(7), 2652-2670.
9. Dawang N D: Phytochemical Constituents and Toxicological Study of Vitex Doniana Leaf. Journal of Pharmacy and Biological Sciences, 2015; 10 (5) 23-27 (safe)
10. Demelash Z K, Asrade S A, Yimer T T, Fentik T B and Berihun B K: Methanolic Crude Extract of Hagenia abyssinica possesses significant antidiarrheal effect: Evidence for Invivo Antidiarrheal Activity. Evidence-Based Complementary and Alternative Medicine 2021.
11. Olubukola S O, Adewale A, Owoade1 A O, Ajayi F A, Adedoyin A A and Adegbola P: Acute and Sub-acute Toxicity Assessment of Euphorbia lateriflora (Schum and Thonn) in Wistar Albino Rats. European Journal of Medicinal Plants, 2019; 29 (1): 1-10.
12. Manaharan T, Srikumar C, Ammu K R, Uma D P (2014): In vivo toxicity evaluation of a standardized extract of Syzygium aqueum leaf Toxicology Reports 1, 2014; 718–725.
13. AssobJCN,KamgaHLF,NsaghaDS, Njunda A L, Nde P F, Asongalem E A, Njouendou A J, Sandjon B and Penlap B V: Antimicrobial and toxicological activities of five medicinal plant speciesfromCameroonTraditional Medicine, Journal of the International Society for Complementary Medicine Research 2011; 11(7).
14. Dzoyem J P, Kuete V and Eloff J N: 23 - Biochemical Parameters in Toxicological Studies in Africa: Significance, Principle of Methods, Data Interpretation and Use in Plant, 2014.
15. Jaykaran P, Bhardwaj N K, Madav P and Panwar P: The internet journal of Toxicology, 2009; (6) 1.
16. Witness M and Admus U L: A Review of Pterocarpus angolensis DC. (Mukwa) an important and Threatened Timber Species of the Miombo Woodlands. Research Journal of Forestry 2016; 10:8-14.
17. Mphande I, Bwalya A G, Muzandu K and Mutaba A: Evaluation of antibacterial activity of Pterocarpus tinctorius methanolic bark extract against enteric bacteria that cause gastroenteritis. Evidence based- Alternative and Complementary medicine, 2022.
18. Tchamadeu M C, Dzeufiet P D D, Nana P, Kouambou N C C, Ngueguim T F, Allardm J, Blaes J, Siagat R, Zapfack L, Girolami J P, Tack I, Kamtchouing P and Dimo T: Acute and sub-chronic oral toxicity studies of an aqueous stem bark extract of Pterocarpus soyauxii Taub (Papillionaceae) in rodent. Journal of Ethnopharmacology, 2011; 133, 329–335.
19. Ihedioha T E, Asuzu I U, Anaga A O and Ikechukwu J I: Hepatoprotective and antioxidant activities of Pterocarpus santalinoides methanol leaf extract. African Journal of Pharmacy and Pharmacology, 2019a; 13(18), 359-373.
20. Maomao. Z, Guangjie. Z, Juan. G, Bo L, Xiaomei. J and Yafang Y: A GC-MS Protocol for Separating
66
Journal of Agriculture and Biomedical Sciences – JABS (2022) | Volume 6 | Issue 1
Endangered and Non-endangered Pterocarpus Wood Species. Molecules, 2019; (24): 799.
21. Lautié E, Russo O, Ducrot P and Boutin J A: Unraveling Plant Natural Chemical Diversity for Drug Discovery Purposes. Frontier Pharmacology, 2020; 11:39.
22. Li D H, Li J Y, Xue C M, Han T, Sai C M, Wang K B et al,: Anti- proliferative dimeric aporphinoid Alkaloids from the Roots of. Journal of Natural Product; 80: 2893-2904.
23. Tumelo A. M, Musonda.D & Chigunta .M (2018). Ethnobotanical Survey of Medical Plants used in Treatment of Diabetes in Chipulukusu Compound, Ndola District, Zambia. Journal of Preventive and Rehabilitative Medicine, 2018; 1(1): 39-44.
24. WHO global report on traditional and complementary medicine: Geneva: World Health Organization, 2019.
25. Chassagne F, Tharanga S, Gina P, James T L, Micah D, Lewis M, Akram M, Shabih S, Darya R F and Quave C L: A Systematic Review of Plants with Antibacterial Activities: A Taxonomic and Phylogenetic Perspective. Journal of Frontiers in Pharmacology, 2021; 11, 2069.
26. World Health Organization: Traditional Medicine Strategy 2014- 2023. WHO Press, World Health Organization, 2013; 20 Avenue Appia, 1211 Geneva 27, Switzerland.
27. Satyajit D S, Zahid L and Alexander I G: Natural Product Isolation, second edition, Humana Press, Totowa, New Jersey, USA, 2006.
28. ShaikhJRandPatilMK:Qualitative tests for preliminary phytochemical screening: An overview, International Journal of Chemical Studies 2020; 8(2), 603-608.
29. OECD: Acute oral toxicity- acute toxic class method. OECD Guidelines for the Testing of Chemicals (No. 423), 2001 (Adopted on 17 December 2011).
30. World Health Organization: General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine, 2000.
31. Porwal M, Khan NA, Maheshwari K K (2017): Evaluation of Acute and Sub-acute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats. Scientia Pharmaceutica, 2017; 85(3): 29.
32. Porwal M, Khan NA, Maheshwari K K (2017): Evaluation of Acute and Sub-acute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats. Scientia Pharmaceutica, 2017; 85(3): 29.
33. Usman M M, Sule M S and Gwarzo M Y: Toxicological studies of aqueous root extract of Euphorbia lateriflora (Schum and Thonn) in rats. Journal of Medicinal Plants Studies, 2014; 2(2): 58-62.
34. Saidu Y, Nwachukwu FC, Bilbis LS, Faruk U.Z and Abbas AY (2010): Toxicity Studies of the Crude Aqueous Root Extract of Albizzia chevalieri Harms in Albino Rats. Nigerian Journal of Basic and Applied Science, 2010; 18(2): 308-314.
35. OECD (2008): Acute Oral Toxicity – Up and Down Procedure (UDP). OECD Guidelines for the Testing of Chemicals (No. 425).
36. Eloff J N: Avoiding pitfalls in determining antimicrobial activity of plant extracts and publishing the results. BMC Complementary and Alternative Medicine, 2019; 19(1).
37. Anshul C, Jasdeep K and Anil K S: Systemic Review: Pharmacognosy, Phytochemistry, Pharmacology and Clinical Applications of Pterocarpus marsupium Roxb. International Journal of Pharmaceutical and Phytopharmacological Research, 2013; 2(5): 319-327.
38. Augustino. S, John B. H, Fortunatus B. S. M. and Romanus C. I: Medicinal Resources of the Miombo woodlands of Urumwa, Tanzania: Plants and its uses. Journal of Medicinal Plants Research, 2011; 5(27): 6352-6372.
39. Convention on International Trade in Endangered Species of wild fauna and flora (CITES): Consideration of proposals for amendment of appendices. Eighteenth meeting, of the Conference of the Parties Colombo (Sri Lanka), 2019
40. Kazhimaika. A: Evaluating crude protein, nitrogen and mineral elements content in pterocarpus chrysothrix (mukula) leaf powder by proximate analysis. Thesis. Copperbelt University, School of Natural Resources, Kapasa Makasa Compass 2018.
41. Vigneshwar R, Arivuchelvan A, Mekala P and Imarayasi M: Sex specific reference interval for Wistar albino rats: hematology and clinical biochemistry, Indian Journal of Animal Health, 2021; 60(1), 58-65
42. Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G, Lilly P, Sanders J, Sipes G, Bracken
W, Dorato M, Van Deun K, Smith P, Berger B, Heller A: Concordance of the toxicity of pharmaceuticals in humans and animals. Regulatory Toxicology and Pharmacology, 2000; 32: 56–67.
43. Tchamadeu M C, Dzeufiet P D D, Nana P, Kouambou N C C, Ngueguim T F, Allardm J, Blaes J, Siagat R, Zapfack L, Girolami J P, Tack I, Kamtchouing P and Dimo T: Acute and sub-chronic oral toxicity studies of an aqueous stem bark extract of Pterocarpus soyauxii Taub (Papillionaceae) in rodent. Journal of Ethnopharmacology, 2011; 133, 329–335.
44. Abdulhafiz F, Farhan Hanif Reduan M, Hamzah Z, Abdul Kari Z, Dawood MAO and Mohammed A: Acute oral toxicity assessment and anti-hyperuricemic activity of Alocasia longiloba extracts on Sprague-Dawley rats. Saudi Journal of Biological Science, 2022; 29(5):3184-3193.
45. Hye-Sun L, Yun S S, Seung M R, Byeong C M, Goya C and Joong-Sun K : “Two-Week Repeated Oral Dose Toxicity Study of Mantidis Ootheca water Extract in C57BL/6 Mice”, Evidence-Based Complementary and Alternative Medicine, 2019.
46. Oladotun A O, Oluwatoyin D M and Olayiwola G: Biochemical, hematological and histopathological evaluation of the toxicity potential of the leaf extract of Stachytarpheta cayennensis in rats, Journal of Traditional and Complementary Medicine, 2020;10 (6).
47. Mohd F H R, Mohd R S, Sayuti N S A, Aliyu A, Md N M M , Bakar Z A and Hamzah H: Evaluation of dermal toxicity study of ethanolic extract of Morinda citrifolia fruit in Spraque Dawley rats. Thailand Journal of Veterinary Medicine, 2021; 51 (1), 101–109.
48. Watthanachaiyingcharoen R, Phanwichien K, Pradermwong A and Kamkaen N (2009): Acute toxicity test of medicinal plants and herbal remedies of aphthous ulcer, Journal of Health Research, (2009); 23(4), 169-174.
49. Sujinda S, Kwanchanok D and Sireewan K: Acute oral toxicological evaluation in Wistar rats of interruptin- rich extract from Cyclosorus terminans and its in vitro antidiabetic potential, Journal of Pharmacy & Pharmacognosy Research, 2022; 10 (5), 800-811, 2022.
50. LeeMY,ShinIS,SeoCS,Kim JH,HaH,HuhJIandShinHK: A 4-week Repeated Dose Oral Toxicity and Cytotoxicity Study of Gumiganghwaltang in Crl: CD (SD) Rats. Toxicology international, 2011; 18(2), 146–154.
51. Ihedioha T E, Asuzu I U, Anaga A O and Ikechukwu J I: Hepatoprotective and antioxidant activities of Pterocarpus santalinoides methanol leaf extract. African Journal of Pharmacy and Pharmacology, 2019a; 13(18), 359-373
52. Ihedioha T E, Odo R I, Onoja US, Nwagu CA, Ihedioha J I (2019 b): Hepatoprotective properties of methanol leaf extract of Pterocarpus mildbraedii Harms on carbon tetrachloride-induced hepatotoxicity in albino rats (Rattus norvegicus). Journal of Complementary Medicine Research 10(4):162-169.
53. Pizzo F, Gadaleta D, Lombardo A, Nicollotti O and Benfenati E : Identification of structural alerts for liver and kidney toxicity using repeated dose toxicity data. Chemistry Central Journal, 2015; 9(62).
54. Singh A, Bhat T K, Sharma O P (2011): Clinical Biochemistry of Hepatotoxicity. Journal of Clinical Toxicology, 2011; 4:1-19.
55. Ping K Y, Darah I, Chen Y, Sreeramanan S and Sasidharan S: Acute and sub-chronic study of Eupthorbia hirta L. methanol extract in rats, Biomedical Research International,2013; 44.
56. Krstic D, Tomic N, Radosavljevic B, Avramovic N, Dragutinovic V, Skodric S R and Colovic M: Biochemical markers of renal function. Current medicinal Chemistry, 2016; 23(19): 2018-2020.
57. Ukwuani AN, Abubaka G, Hassan SW, Agaie BM: Toxicological studies of hydromel thanolic leaves extract of Grewia crenata. International Journal of Pharmaceutical Sciences and Drug Research, 2012; 4 (4):245-249.
58. Kazi M D, Mahmudul H, Nasrin T, Anwarul H: Biochemical and histopathological profiling of Wistar rat treated with Brassica napus as a supplementary feed, Food Science and Human Wellness, 2018; 7(1), 77-82.
59. Wang D, Xu K, Zhong Y, Luo X, Xiao R, Y. Hou, Bao W, Yang W, Yan H, Yao P and Liu L: Acute and sub-chronic oral toxicities of Pu- erh black tea extract in Sprague- Dawley rats, Journal of Ethno- pharmacology, 2011; 134, 156–164.
60. Kampfmann I, Bauer N, Johannes S, Moritz A: Differences in hematologic variables in rats of the same strain but different origin. Journal of Veterinary Clinical Pathology, 2012; 41(2), 228-234.
61. He Q, Su G, Liu K, Zhang F, Jiang Y, Gao J, et al, : Sex-specific reference intervals of hematologic and biochemical analytes in Sprague- Dawley rats using the nonparametric rank percentile method. PLoS ONE, 2017; 12(12).
62. Atsamo A D, Nguelefack T B, Datte J Y and Kamanyi A: Acute and sub- chronic oral toxicity assessment of the aqueous extract from the stem bark of Erythrina senegalensis DC (Fabaceae) in rodents, Journal Ethno-pharmacology, 2011; 34, 697–702.
63. Thammitiyagodage M G, de Silva NR,RathnayakeC,Karunakaran R, Kumara WGSS and Gunatillka M M et al,: Biochemical and histopathological changes in Wistar rats after consumption of boiled and un-boiled water from high and low diseaseprevalentareasforchronic kidney disease of unknown etiology (CKDu) in north Central Province (NCP) and its comparison with low disease prevalent Colombo, Sri Lanka BMC Nephrology, 2020; 21(38).
64. Djabir Y Y, Arsyad M A, Sartini S and Lallo S: Potential Roles of Kleinhovia hospita L. Leaf Extract in Reducing Doxorubicin Acute Hepatic, Cardiac and Renal Toxicities in Rats. Pharmacognosy research 2017; 9 (2): 168–173 https:// doi.org/10.4103/pr.pr_129_16.
65. Moriles K E and Azer S A: Alanine Amino Transferase [updated, 2021, May 9]. In-Stat Pearls [Internet], Treasure Island (FL): Stat Pearls publishing, 2021.
66. Ihedioha TE, Onwuegbuka LU and Ihedioha JI: Hepatoprotective effects of methanol leaf extract of Pterocarpus santalinoides DC on acetaminophen-induced liver damage in albino rats (Rattus norvegicus). Nigerian Journal of Animal Production; 2017; 44 (5): 34-39.
67. IhediohaTE,OkechukuVN,Ihedioha J I: Effects of aqueous leaf infusion of Pterocarpus santalinoides D C on serum lipid profile of guinea pigs (Carvia porcellus). Journal of Complementary Medicine Research 2018; 7 (2): 154-160.
68. Giknis M L A and Clifford CB (2008): Clinical laboratory parameters for Crl: WI (Han) rats, Charles River 251 Ballardvalle Street, Wilmington, MA 01887, 781-222-6000.
69. Division of Laboratory Animal Medicine: Serum Chemistry Reference range, Pathology and Laboratory Medicine Services, David Geffen School of Medi- cine at UCLA 650 Charles E. Young Drive South, CHS:5V-109 Los Angeles, CA 90095-1718 ph: (310) 206-8120, 2013.
2. Nasri H and Shirzad H: Toxicity and safety of medicinal plants. Journal of HerbMed Pharmacology 2013; 2(2): 21-22.
3. Botha CJ and Penrith ML: Poisonous plants of veterinary and human importance in Southern Africa, Journal of Ethnopharmacology 2008; 119, 549-58.
4. Kalaiselvi P, Uma M, Suresh M, Thulasiraman K and Lakshmidevi E: Chronic toxicity studies of aqueous leaf extract of Indian traditional medicinal plant Ocimum tenuiflorum (Linn.) in rats European. Journal of Experimental Biology 2013; (5): 240-247.
5. Fred-Jaiyesimi A A, Ajibesin K K: Ethnobotanical survey of toxic plants and plant parts in Ogun State, Nigeria. International Journal of Green Pharmacy 2012; 6: 174-9.
6. Atchou K, Lawson-Evi P and Eklu- Gadegbeku K: Safety assessment of the dried hydroethanolic extracts of Pterocarpus erinaceus Poir. Stem bark, Phytomedicine Plus 2021; 1(4).
7. Salawu O A, Aliyu M and Tijani AY: Hematological studies on the ethanolic stem bark extract of Pterocarpus erinaceous Poir (Fabaceae). African Journal of Biotechnology 2008; 7(9).
8. Chanda J, Parekh J, Vaghasiya Y, Dave R, Baravalia Y and Nair R: Medicinal plants- from-from Traditional use to toxicity assessment: A Review- International Journal of Pharmaceutical Sciences and Research 2015; 6(7), 2652-2670.
9. Dawang N D: Phytochemical Constituents and Toxicological Study of Vitex Doniana Leaf. Journal of Pharmacy and Biological Sciences, 2015; 10 (5) 23-27 (safe)
10. Demelash Z K, Asrade S A, Yimer T T, Fentik T B and Berihun B K: Methanolic Crude Extract of Hagenia abyssinica possesses significant antidiarrheal effect: Evidence for Invivo Antidiarrheal Activity. Evidence-Based Complementary and Alternative Medicine 2021.
11. Olubukola S O, Adewale A, Owoade1 A O, Ajayi F A, Adedoyin A A and Adegbola P: Acute and Sub-acute Toxicity Assessment of Euphorbia lateriflora (Schum and Thonn) in Wistar Albino Rats. European Journal of Medicinal Plants, 2019; 29 (1): 1-10.
12. Manaharan T, Srikumar C, Ammu K R, Uma D P (2014): In vivo toxicity evaluation of a standardized extract of Syzygium aqueum leaf Toxicology Reports 1, 2014; 718–725.
13. AssobJCN,KamgaHLF,NsaghaDS, Njunda A L, Nde P F, Asongalem E A, Njouendou A J, Sandjon B and Penlap B V: Antimicrobial and toxicological activities of five medicinal plant speciesfromCameroonTraditional Medicine, Journal of the International Society for Complementary Medicine Research 2011; 11(7).
14. Dzoyem J P, Kuete V and Eloff J N: 23 - Biochemical Parameters in Toxicological Studies in Africa: Significance, Principle of Methods, Data Interpretation and Use in Plant, 2014.
15. Jaykaran P, Bhardwaj N K, Madav P and Panwar P: The internet journal of Toxicology, 2009; (6) 1.
16. Witness M and Admus U L: A Review of Pterocarpus angolensis DC. (Mukwa) an important and Threatened Timber Species of the Miombo Woodlands. Research Journal of Forestry 2016; 10:8-14.
17. Mphande I, Bwalya A G, Muzandu K and Mutaba A: Evaluation of antibacterial activity of Pterocarpus tinctorius methanolic bark extract against enteric bacteria that cause gastroenteritis. Evidence based- Alternative and Complementary medicine, 2022.
18. Tchamadeu M C, Dzeufiet P D D, Nana P, Kouambou N C C, Ngueguim T F, Allardm J, Blaes J, Siagat R, Zapfack L, Girolami J P, Tack I, Kamtchouing P and Dimo T: Acute and sub-chronic oral toxicity studies of an aqueous stem bark extract of Pterocarpus soyauxii Taub (Papillionaceae) in rodent. Journal of Ethnopharmacology, 2011; 133, 329–335.
19. Ihedioha T E, Asuzu I U, Anaga A O and Ikechukwu J I: Hepatoprotective and antioxidant activities of Pterocarpus santalinoides methanol leaf extract. African Journal of Pharmacy and Pharmacology, 2019a; 13(18), 359-373.
20. Maomao. Z, Guangjie. Z, Juan. G, Bo L, Xiaomei. J and Yafang Y: A GC-MS Protocol for Separating
66
Journal of Agriculture and Biomedical Sciences – JABS (2022) | Volume 6 | Issue 1
Endangered and Non-endangered Pterocarpus Wood Species. Molecules, 2019; (24): 799.
21. Lautié E, Russo O, Ducrot P and Boutin J A: Unraveling Plant Natural Chemical Diversity for Drug Discovery Purposes. Frontier Pharmacology, 2020; 11:39.
22. Li D H, Li J Y, Xue C M, Han T, Sai C M, Wang K B et al,: Anti- proliferative dimeric aporphinoid Alkaloids from the Roots of. Journal of Natural Product; 80: 2893-2904.
23. Tumelo A. M, Musonda.D & Chigunta .M (2018). Ethnobotanical Survey of Medical Plants used in Treatment of Diabetes in Chipulukusu Compound, Ndola District, Zambia. Journal of Preventive and Rehabilitative Medicine, 2018; 1(1): 39-44.
24. WHO global report on traditional and complementary medicine: Geneva: World Health Organization, 2019.
25. Chassagne F, Tharanga S, Gina P, James T L, Micah D, Lewis M, Akram M, Shabih S, Darya R F and Quave C L: A Systematic Review of Plants with Antibacterial Activities: A Taxonomic and Phylogenetic Perspective. Journal of Frontiers in Pharmacology, 2021; 11, 2069.
26. World Health Organization: Traditional Medicine Strategy 2014- 2023. WHO Press, World Health Organization, 2013; 20 Avenue Appia, 1211 Geneva 27, Switzerland.
27. Satyajit D S, Zahid L and Alexander I G: Natural Product Isolation, second edition, Humana Press, Totowa, New Jersey, USA, 2006.
28. ShaikhJRandPatilMK:Qualitative tests for preliminary phytochemical screening: An overview, International Journal of Chemical Studies 2020; 8(2), 603-608.
29. OECD: Acute oral toxicity- acute toxic class method. OECD Guidelines for the Testing of Chemicals (No. 423), 2001 (Adopted on 17 December 2011).
30. World Health Organization: General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine, 2000.
31. Porwal M, Khan NA, Maheshwari K K (2017): Evaluation of Acute and Sub-acute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats. Scientia Pharmaceutica, 2017; 85(3): 29.
32. Porwal M, Khan NA, Maheshwari K K (2017): Evaluation of Acute and Sub-acute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats. Scientia Pharmaceutica, 2017; 85(3): 29.
33. Usman M M, Sule M S and Gwarzo M Y: Toxicological studies of aqueous root extract of Euphorbia lateriflora (Schum and Thonn) in rats. Journal of Medicinal Plants Studies, 2014; 2(2): 58-62.
34. Saidu Y, Nwachukwu FC, Bilbis LS, Faruk U.Z and Abbas AY (2010): Toxicity Studies of the Crude Aqueous Root Extract of Albizzia chevalieri Harms in Albino Rats. Nigerian Journal of Basic and Applied Science, 2010; 18(2): 308-314.
35. OECD (2008): Acute Oral Toxicity – Up and Down Procedure (UDP). OECD Guidelines for the Testing of Chemicals (No. 425).
36. Eloff J N: Avoiding pitfalls in determining antimicrobial activity of plant extracts and publishing the results. BMC Complementary and Alternative Medicine, 2019; 19(1).
37. Anshul C, Jasdeep K and Anil K S: Systemic Review: Pharmacognosy, Phytochemistry, Pharmacology and Clinical Applications of Pterocarpus marsupium Roxb. International Journal of Pharmaceutical and Phytopharmacological Research, 2013; 2(5): 319-327.
38. Augustino. S, John B. H, Fortunatus B. S. M. and Romanus C. I: Medicinal Resources of the Miombo woodlands of Urumwa, Tanzania: Plants and its uses. Journal of Medicinal Plants Research, 2011; 5(27): 6352-6372.
39. Convention on International Trade in Endangered Species of wild fauna and flora (CITES): Consideration of proposals for amendment of appendices. Eighteenth meeting, of the Conference of the Parties Colombo (Sri Lanka), 2019
40. Kazhimaika. A: Evaluating crude protein, nitrogen and mineral elements content in pterocarpus chrysothrix (mukula) leaf powder by proximate analysis. Thesis. Copperbelt University, School of Natural Resources, Kapasa Makasa Compass 2018.
41. Vigneshwar R, Arivuchelvan A, Mekala P and Imarayasi M: Sex specific reference interval for Wistar albino rats: hematology and clinical biochemistry, Indian Journal of Animal Health, 2021; 60(1), 58-65
42. Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G, Lilly P, Sanders J, Sipes G, Bracken
W, Dorato M, Van Deun K, Smith P, Berger B, Heller A: Concordance of the toxicity of pharmaceuticals in humans and animals. Regulatory Toxicology and Pharmacology, 2000; 32: 56–67.
43. Tchamadeu M C, Dzeufiet P D D, Nana P, Kouambou N C C, Ngueguim T F, Allardm J, Blaes J, Siagat R, Zapfack L, Girolami J P, Tack I, Kamtchouing P and Dimo T: Acute and sub-chronic oral toxicity studies of an aqueous stem bark extract of Pterocarpus soyauxii Taub (Papillionaceae) in rodent. Journal of Ethnopharmacology, 2011; 133, 329–335.
44. Abdulhafiz F, Farhan Hanif Reduan M, Hamzah Z, Abdul Kari Z, Dawood MAO and Mohammed A: Acute oral toxicity assessment and anti-hyperuricemic activity of Alocasia longiloba extracts on Sprague-Dawley rats. Saudi Journal of Biological Science, 2022; 29(5):3184-3193.
45. Hye-Sun L, Yun S S, Seung M R, Byeong C M, Goya C and Joong-Sun K : “Two-Week Repeated Oral Dose Toxicity Study of Mantidis Ootheca water Extract in C57BL/6 Mice”, Evidence-Based Complementary and Alternative Medicine, 2019.
46. Oladotun A O, Oluwatoyin D M and Olayiwola G: Biochemical, hematological and histopathological evaluation of the toxicity potential of the leaf extract of Stachytarpheta cayennensis in rats, Journal of Traditional and Complementary Medicine, 2020;10 (6).
47. Mohd F H R, Mohd R S, Sayuti N S A, Aliyu A, Md N M M , Bakar Z A and Hamzah H: Evaluation of dermal toxicity study of ethanolic extract of Morinda citrifolia fruit in Spraque Dawley rats. Thailand Journal of Veterinary Medicine, 2021; 51 (1), 101–109.
48. Watthanachaiyingcharoen R, Phanwichien K, Pradermwong A and Kamkaen N (2009): Acute toxicity test of medicinal plants and herbal remedies of aphthous ulcer, Journal of Health Research, (2009); 23(4), 169-174.
49. Sujinda S, Kwanchanok D and Sireewan K: Acute oral toxicological evaluation in Wistar rats of interruptin- rich extract from Cyclosorus terminans and its in vitro antidiabetic potential, Journal of Pharmacy & Pharmacognosy Research, 2022; 10 (5), 800-811, 2022.
50. LeeMY,ShinIS,SeoCS,Kim JH,HaH,HuhJIandShinHK: A 4-week Repeated Dose Oral Toxicity and Cytotoxicity Study of Gumiganghwaltang in Crl: CD (SD) Rats. Toxicology international, 2011; 18(2), 146–154.
51. Ihedioha T E, Asuzu I U, Anaga A O and Ikechukwu J I: Hepatoprotective and antioxidant activities of Pterocarpus santalinoides methanol leaf extract. African Journal of Pharmacy and Pharmacology, 2019a; 13(18), 359-373
52. Ihedioha T E, Odo R I, Onoja US, Nwagu CA, Ihedioha J I (2019 b): Hepatoprotective properties of methanol leaf extract of Pterocarpus mildbraedii Harms on carbon tetrachloride-induced hepatotoxicity in albino rats (Rattus norvegicus). Journal of Complementary Medicine Research 10(4):162-169.
53. Pizzo F, Gadaleta D, Lombardo A, Nicollotti O and Benfenati E : Identification of structural alerts for liver and kidney toxicity using repeated dose toxicity data. Chemistry Central Journal, 2015; 9(62).
54. Singh A, Bhat T K, Sharma O P (2011): Clinical Biochemistry of Hepatotoxicity. Journal of Clinical Toxicology, 2011; 4:1-19.
55. Ping K Y, Darah I, Chen Y, Sreeramanan S and Sasidharan S: Acute and sub-chronic study of Eupthorbia hirta L. methanol extract in rats, Biomedical Research International,2013; 44.
56. Krstic D, Tomic N, Radosavljevic B, Avramovic N, Dragutinovic V, Skodric S R and Colovic M: Biochemical markers of renal function. Current medicinal Chemistry, 2016; 23(19): 2018-2020.
57. Ukwuani AN, Abubaka G, Hassan SW, Agaie BM: Toxicological studies of hydromel thanolic leaves extract of Grewia crenata. International Journal of Pharmaceutical Sciences and Drug Research, 2012; 4 (4):245-249.
58. Kazi M D, Mahmudul H, Nasrin T, Anwarul H: Biochemical and histopathological profiling of Wistar rat treated with Brassica napus as a supplementary feed, Food Science and Human Wellness, 2018; 7(1), 77-82.
59. Wang D, Xu K, Zhong Y, Luo X, Xiao R, Y. Hou, Bao W, Yang W, Yan H, Yao P and Liu L: Acute and sub-chronic oral toxicities of Pu- erh black tea extract in Sprague- Dawley rats, Journal of Ethno- pharmacology, 2011; 134, 156–164.
60. Kampfmann I, Bauer N, Johannes S, Moritz A: Differences in hematologic variables in rats of the same strain but different origin. Journal of Veterinary Clinical Pathology, 2012; 41(2), 228-234.
61. He Q, Su G, Liu K, Zhang F, Jiang Y, Gao J, et al, : Sex-specific reference intervals of hematologic and biochemical analytes in Sprague- Dawley rats using the nonparametric rank percentile method. PLoS ONE, 2017; 12(12).
62. Atsamo A D, Nguelefack T B, Datte J Y and Kamanyi A: Acute and sub- chronic oral toxicity assessment of the aqueous extract from the stem bark of Erythrina senegalensis DC (Fabaceae) in rodents, Journal Ethno-pharmacology, 2011; 34, 697–702.
63. Thammitiyagodage M G, de Silva NR,RathnayakeC,Karunakaran R, Kumara WGSS and Gunatillka M M et al,: Biochemical and histopathological changes in Wistar rats after consumption of boiled and un-boiled water from high and low diseaseprevalentareasforchronic kidney disease of unknown etiology (CKDu) in north Central Province (NCP) and its comparison with low disease prevalent Colombo, Sri Lanka BMC Nephrology, 2020; 21(38).
64. Djabir Y Y, Arsyad M A, Sartini S and Lallo S: Potential Roles of Kleinhovia hospita L. Leaf Extract in Reducing Doxorubicin Acute Hepatic, Cardiac and Renal Toxicities in Rats. Pharmacognosy research 2017; 9 (2): 168–173 https:// doi.org/10.4103/pr.pr_129_16.
65. Moriles K E and Azer S A: Alanine Amino Transferase [updated, 2021, May 9]. In-Stat Pearls [Internet], Treasure Island (FL): Stat Pearls publishing, 2021.
66. Ihedioha TE, Onwuegbuka LU and Ihedioha JI: Hepatoprotective effects of methanol leaf extract of Pterocarpus santalinoides DC on acetaminophen-induced liver damage in albino rats (Rattus norvegicus). Nigerian Journal of Animal Production; 2017; 44 (5): 34-39.
67. IhediohaTE,OkechukuVN,Ihedioha J I: Effects of aqueous leaf infusion of Pterocarpus santalinoides D C on serum lipid profile of guinea pigs (Carvia porcellus). Journal of Complementary Medicine Research 2018; 7 (2): 154-160.
68. Giknis M L A and Clifford CB (2008): Clinical laboratory parameters for Crl: WI (Han) rats, Charles River 251 Ballardvalle Street, Wilmington, MA 01887, 781-222-6000.
69. Division of Laboratory Animal Medicine: Serum Chemistry Reference range, Pathology and Laboratory Medicine Services, David Geffen School of Medi- cine at UCLA 650 Charles E. Young Drive South, CHS:5V-109 Los Angeles, CA 90095-1718 ph: (310) 206-8120, 2013.
Published
2022-09-27
How to Cite
1.
Mphande I, Kataba A, Muzandu K, Bwalya A. Phytochemical Screening and Acute Exposure of Methanolic Bark Extract of Pterocarpus Tinctorius in Wistar Rats. Journal of Agricultural and Biomedical Sciences [Internet]. 27Sep.2022 [cited 24Nov.2024];6(1). Available from: https://humanities.unza.zm/index.php/JABS/article/view/873
Section
Biomedical Sciences
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